Generator

Part:BBa_K559010:Design

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_CUHK   (2011-09-29)

Halorhodopsin complete system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 380
    Illegal AgeI site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 316


Design Notes

The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system


Usage and Biology

See BBa_K559000 for additional details.

Source

Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)

References